cd177 antibody (4c4)  (Bio-Techne corporation)

Journal: BMC Gastroenterology

Article Title: Gene expression analysis of a Helicobacter pylori -infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer

doi: 10.1186/1471-230X-13-122

Figure Lengend Snippet: Primer sequences for relative quantitative real-time RT-PCR

Article Snippet: Then, sections were incubated with a mouse monoclonal anti-CD177 antibody (clone 4C4, diluted 1:100, Abnova, Taipei, Taiwan).

Techniques:

Journal: BMC Gastroenterology

Article Title: Gene expression analysis of a Helicobacter pylori -infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer

doi: 10.1186/1471-230X-13-122

Figure Lengend Snippet: Global gene analysis in the glandular stomach of MNU-treated mice using oligonucleotide microarray. A : Number of genes up- or down-regulated more than two-fold in the stomach of MNU-treated mice. In Venn’s diagram, the circles indicate up- (left) or down-regulated (right) genes in the stomach of MNU-treated mice with H. pylori infection, high-salt diet or their combination. The shaded area represents the up- or down-regulated genes more than two-fold only by the combination. B : Quantitative real-time RT-PCR analysis of three selected up-regulated genes ( Cd177 , Reg3g , and Muc13 ) in the stomachs of MNU-treated mice. Expression levels of the genes in each sample were normalized by Gapdh as internal control using ΔΔCT method. Relative expression levels were represented as the X-fold change relative to Group A (fixed as 1.0). Statistical analysis was performed by the Kruskal-Wallis test for general analysis and Tukey test for multiple comparison. Bars, SE; *, P < 0.01 vs. Group A and < 0.05 vs. Group C; †, P < 0.01 vs. Group C.

Article Snippet: Then, sections were incubated with a mouse monoclonal anti-CD177 antibody (clone 4C4, diluted 1:100, Abnova, Taipei, Taiwan).

Techniques: Microarray, Infection, Quantitative RT-PCR, Expressing, Control, Comparison

Journal: BMC Gastroenterology

Article Title: Gene expression analysis of a Helicobacter pylori -infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer

doi: 10.1186/1471-230X-13-122

Figure Lengend Snippet: CD177 expression in gastric carcinomas and its correlation with clinicopathological factors

Article Snippet: Then, sections were incubated with a mouse monoclonal anti-CD177 antibody (clone 4C4, diluted 1:100, Abnova, Taipei, Taiwan).

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: Gene expression analysis of a Helicobacter pylori -infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer

doi: 10.1186/1471-230X-13-122

Figure Lengend Snippet: Multivariate analysis of prognostic factos in patients with gastric cancer using Cox proportional hazard model

Article Snippet: Then, sections were incubated with a mouse monoclonal anti-CD177 antibody (clone 4C4, diluted 1:100, Abnova, Taipei, Taiwan).

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: Gene expression analysis of a Helicobacter pylori -infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer

doi: 10.1186/1471-230X-13-122

Figure Lengend Snippet: Regulated genes by combination of H. pylori infection and high-salt diet in mouse gastric mucosa

Article Snippet: Then, sections were incubated with a mouse monoclonal anti-CD177 antibody (clone 4C4, diluted 1:100, Abnova, Taipei, Taiwan).

Techniques: Infection, Transformation Assay, Membrane, Retroviral, Sequencing